Candidate and study goal
Define the target, mechanism and development stage, then determine whether the study will screen or rank candidates or investigate the basis of response.
Application
Small-molecule & targeted therapy studies
Compare candidates across doses, time points and combinations in models selected for the target and indication, using mechanism-linked readouts to explain differences.
Model context and treatment response
Connect model identity, dose response and mechanism readouts
Glioma PDO 2.0 evidence pairs source-tissue/PDO histology and lineage profiling with apoptosis and necrosis across inhibitor concentrations. New studies can extend this approach to candidate-specific dose, timing, combination and mechanism readouts.
View imageModel and lineage context: source-tissue/PDO histology with Nestin, Olig2 and S100B profiling establishes glioma-model features for candidate and mechanism studies.
TRiCBIO glioma PDO 2.0 study data
View imageTreatment response: early-apoptosis and necrosis readouts across inhibitor concentrations show dose-related changes and guide replication, combination and mechanism studies.
TRiCBIO glioma PDO 2.0 study dataReview the full study
View the glioma PDO 2.0 study Continue to the related case for model context, experimental setup and interpretation.
Study focus
What this study can help you decide
- 01What dose-response profile does the candidate show?
- 02What explains differences across models or samples?
- 03What does the combination add over either monotherapy?
Study workflow
How the study moves from question to results
Model and assay window
Select a biologically relevant model and confirm model performance, vehicle tolerance, positive-control response and assay window.
Dose, time and combinations
Use dose series, time points or combination matrices with consistent viability, morphology and mechanism-linked readouts.
Confirmation and ranking
Confirm initial hits and rank candidates using potency, maximum effect, selectivity and data quality.
Model options
Match the model to indication, mechanism and sample
Plate format, throughput, dose range and hit criteria are adapted to the model and candidate; in vitro results support candidate comparison and the design of follow-on in vivo validation.
PROJECT DISCUSSION
Plan a small-molecule or targeted therapy study
The target, indication, candidate set and current dose, combination or mechanism question guide model selection, controls and study endpoints.