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Organoid efficacy screening

Compare single agents, dose responses and combinations in selected organoid models to identify dose-dependent and model-specific candidate responses.

Dose-related morphology in two breast cancer PDO models from a plate-based ADC studyView image
TRiCBIO breast cancer PDO ADC study data

Breast cancer PDOs · plate-based ADC study

Establish a plate-based baseline with ADC dose response, then extend to candidate comparison

This ADC study illustrates a plate-based workflow across two breast cancer PDO models, comparing control and dose conditions with model-specific response curves. The same design can be adapted to small molecules, combinations and other modalities, with endpoints selected for their mechanism.

Study focus

What this study can help you decide

  1. 01How does response change across dose?
  2. 02What does the combination add over either single agent?
  3. 03How does response vary across models or samples?

STUDY APPROACH

Match the model and assays to your program

Suitable projects

Programs comparing candidates, dose, time or combinations to establish a basis for prioritization.

Model strategy

Conventional PDOs suit many tumor-epithelial efficacy comparisons; assess PDO 2.0 or a relevant co-culture when tissue, immune or spatial context is central.

Assay set

Combine viability, morphology, apoptosis, imaging and mechanism-linked endpoints around the candidate modality and expected differences.

Key controls

Define vehicle and positive controls, replication, assay window and prespecified comparison or hit criteria.

How results inform your program

Prioritize candidates, dose windows and combinations, then define the next orthogonal or mechanism study.

Study workflow

Compare and rank candidates in consistent organoid studies

Confirm model batch quality, assay window and readout stability, then design dose, timing, combinations and mechanism-linked endpoints to produce comparable screening results.

01

Screen objective and candidate tiers

Define primary screening, confirmation, dose ranking or combination comparison, then stage the work by modality, candidate count and available evidence.

02

Model batch and assay window

Confirm identity, viability, well-to-well consistency, vehicle tolerance and positive-control response before expanding the screen.

03

Dose, combinations and multimodal readouts

Use single-agent dose series, combination matrices or longitudinal observation, interpreting viability alongside morphology, apoptosis, imaging or molecular readouts.

04

Confirmation and candidate ranking

Confirm preliminary hits with independent repeats or complementary endpoints, then rank candidates using potency, maximum effect, selectivity and data quality.

Expected study outputs

What you receive

01

Comparable response curves

Single agents, dose series or combination matrices use consistent experimental and analytical criteria.

02

Mechanism-linked readouts

An integrated analysis of viability, morphology, imaging, molecular or secreted readouts selected around the mechanism of action.

03

A traceable study report

Experimental conditions, model QC, raw data, analysis methods and key findings in a single study report.

Relevant model systems

Recommended model systems

Study design

Plate format and study scale are adapted to the model, candidate count and endpoints, with sample count, replicates, assay window and evaluation criteria defined before the study begins.

PROJECT DISCUSSION

Plan an organoid efficacy study

Bring the candidate set, dose or combination plan, intended models and key endpoints. We’ll define the plate format, controls, replicates and comparison strategy with you.