Target and tissue context
Use histology, IHC or IF to assess target expression and spatial heterogeneity, and confirm model identity and culture quality.
Application
ADC efficacy & tissue distribution
Compare ADC candidates in human organoids using target-expression, tissue-distribution and activity readouts, with dedicated studies of bystander killing.
Spatial imaging and activity studies
Antibody distribution imaging and ADC activity
Breast cancer PDO studies combine frozen-section distribution of AF549-labeled antibody/nanobody formats with morphology and viability after ADC treatment. A new candidate program can add target profiling, imaging controls, dedicated penetration quantification and killing endpoints.
View imageFrozen-section spatial distribution: bright-field, DAPI, AF549 and merged views compare relative signal patterns for labeled antibody and nanobody in PDO frozen sections.
TRiCBIO breast cancer PDO antibody/nanobody distribution study
View imagePost-treatment morphology: bright-field views under control, 0.3 μM and 3.0 μM conditions provide tissue context for viability readouts.
TRiCBIO breast cancer PDO ADC study data
View imageDose response: two PDO models show different viability profiles across a candidate ADC concentration range, informing replication and follow-on sample studies.
TRiCBIO breast cancer PDO ADC study dataReview the full study
View antibody distribution and ADC response Continue to the related case for model context, experimental setup and interpretation.
Study focus
What this study can help you decide
- 01How do target level and spatial pattern support candidate comparison?
- 02How do tissue distribution and killing relate?
- 03When are penetration, bystander or immune endpoints valuable?
Study workflow
How the study moves from question to results
Labeling and spatial imaging
Define labeling, incubation, imaging and competition or target-negative controls around molecule properties; add dedicated imaging and quantification when intact-organoid penetration is a priority.
Killing and mechanism endpoints
Interpret spatial distribution with dose response, viability, apoptosis and histology.
ADC ranking
Compare candidates consistently and deliver original images, quantitative analysis and interpretation.
Model options
Match the model to indication, mechanism and sample
ADC studies first compare candidates through section imaging and functional readouts; quantitative penetration studies can then add serial sections or z-stacks, distance/intensity profiles, time points and target/competition controls.
PROJECT DISCUSSION
Plan an ADC organoid study
ADC design and target biology determine the tumor models, distribution imaging, killing and bystander endpoints for the study.