01Source tissue
Source-tissue H&E morphology
Source-tissue H&E provides the histological reference for subsequent PDO morphology and histology.
Establishes a histologic reference for PDO quality assessmentModel & assay dataCase study
Breast cancer PDO: phenotype & tissue identity
Assess ER and Ki67 phenotypes alongside source-tissue/PDO histology to establish model identity and select efficacy endpoints.
01Study objective02Model & design03Assays & findings04Decision & next step
Model & sampleBreast cancer PDO with source-tissue comparison
ObjectiveCompare source-tissue and PDO morphology and establish subtype-associated phenotype before efficacy work
Study conditionsModel identity and baseline characterization
AssaysBright-field, H&E, DAPI, ER and Ki67
Study evidenceTRiCBIO culture and characterization images
DeliverablesReceive images, assay results and an analysis summary; the report documents the study design, sample size and statistical methods
SUBTYPE & TISSUE IDENTITY
Define subtype and tissue identity
ER and Ki67 show receptor-associated and proliferative phenotypes; source-tissue and PDO morphology and histology then guide model and endpoint selection for subtype-focused efficacy studies.
View imageReceptor and proliferation phenotype: DAPI, ER and Ki67 show nuclear, receptor-associated and proliferative features in this breast PDO and provide a baseline for subtype-informed study design.
Model & assay dataSTUDY EVIDENCE
Source tissue, PDO morphology and histology
Source-tissue H&E, PDO bright-field imaging and PDO histology establish morphological correspondence and inform receptor, proliferation and target profiling.
02Culture morphology
PDO structure in 3D culture
Bright-field imaging documents 3D growth morphology before histology and downstream treatment studies.
Shows culture status before further profiling03PDO histology
Tissue structure in the PDO section
PDO H&E is interpreted with the source-tissue reference to assess morphology; molecular concordance is evaluated with markers and omics assays selected for the study.
Informs receptor, proliferation and target assays
INTERPRETATION
Source-tissue and PDO morphology with subtype features
Source-tissue and PDO morphological comparisons plus receptor profiling clarify sample context and model subtype phenotype.
Morphological correspondence, receptor phenotype and culture state support model selection, with long-term stability confirmed through passage and batch QC.
Use subtype and target context to select the most relevant efficacy, ADC, combination or mechanism study.
Related models and services
Choose the right models and services for the next study
Study planning
Build your study from this evidence
Model characterization, subtype-focused studies or candidate evaluation
Pathology subtype, sample context, target background and downstream objective
Culture and tissue comparison, subtype-associated profiling and model selection for the intended study
Bright-field and histology images, marker results, QC assessment and recommended study conditions
Define the subtype- or target-informed study
Receptor status, culture stability, candidate mechanism and inter-sample variation
PROJECT DISCUSSION
Discuss a study built around your program
Tell us which model, assay or finding you want to extend. Our scientists will adapt the approach to your candidate, samples and decision point.