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Application

Cell & gene therapy studies

Use human organoids for gene delivery, genetic perturbation and co-culture studies with therapeutic cells, with functional, mechanistic and safety readouts matched to the program.

Organoid gene-delivery and perturbation workflow from model selection to functional validation
Gene delivery and perturbation workflow

Two study approaches

Match gene delivery or therapeutic-cell function to the target model

The map shows the gene-delivery and perturbation workflow. Therapeutic-cell studies use a tailored co-culture based on cell type, effector–target context and tissue response; both approaches can extend into functional, mechanistic and safety assays.

Gene delivery & perturbationModel fit → delivery & expression → molecular confirmation → functional phenotype
Therapeutic-cell function & co-cultureCandidate cells → effector–target setup → tissue response → safety endpoints

STUDY IMAGES

Lentiviral delivery and expression in HCC organoids

Day-3 bright-field and fluorescence images show lentiviral expression under control and lncRNA shRNA conditions, with molecular, phenotypic and treatment-response assays available for follow-on studies.

HCC organoids · Scramble shRNA control · Day 3View image
01Scramble shRNA control · Day 3

Bright-field and green-fluorescence overlay showing organoid morphology and expression in the control condition.

Study results
HCC organoids · lncRNA shRNA condition · Day 3View image
02lncRNA shRNA condition · Day 3

Organoid morphology and expression at the same time point, shown alongside the control condition.

Study results

Study focus

What this study can help you decide

  1. 01Is the target model suitable for the proposed delivery or genetic perturbation?
  2. 02What functional phenotype does the intervention produce?
  3. 03When should organ-safety readouts be added?

Study workflow

How the study moves from question to results

01

Vector, cell and model setup

Define vector or cell format, target cell, model integrity and key assay endpoints.

02

Delivery and expression assessment

Assess entry, expression, perturbation performance and model tolerance under defined conditions.

03

Function and mechanism

After establishing delivery and perturbation performance, assess the intended phenotype, pathway and durability.

04

Safety and sample expansion

Add normal-tissue, inflammatory or off-target readouts around the mechanism, then plan work in additional samples.

Model options

Match the model to indication, mechanism and sample

Study design options

Co-development aligns delivery, perturbation performance, model QC and functional endpoints, then extends the results into mechanism or safety studies.

PROJECT DISCUSSION

Plan a cell or gene therapy study

Vector or cell format, target tissue and intended functional phenotype guide model selection, perturbation assessment and safety readouts.