Culture records, epithelial identity and stage-associated markers characterize model establishment and the different culture stages.
Model & assay dataModel characterization
Endometrial organoid profiling
Evaluate primary establishment and passage with H&E, E-cadherin and Ki67, plus ER, PR and FOXO1 profiling, to assess identity and stage-dependent change.
01Study objective02Model & design03Assays & findings04Decision & next step
Model & sampleHuman tissue-derived endometrial organoids
ObjectiveEstablish model growth, epithelial identity and stage characterization
Study conditionsProliferative, secretory and receptive culture stages
AssaysBright-field, H&E, E-cadherin, Ki67, ER, PR and FOXO1
Study evidenceTRiCBIO endometrial organoid establishment and stage-characterization data
DeliverablesReceive images, assay results and an analysis summary; the report documents the study design, sample size and statistical methods
ESTABLISHMENT & STAGE PHENOTYPES
Stage-specific endometrial phenotypes
Serial culture, H&E, E-cadherin and Ki67 establish model growth and epithelial state; ER, PR and FOXO1 then show phenotype changes across culture stages.
View imageEstablishment and passage: the displayed time points document organoid formation after primary processing and continued growth after passage.
TRiCBIO human endometrial organoid characterization data
View imageBaseline identity: H&E, DAPI, E-cadherin and Ki67 images compare source tissue and organoids for epithelial organization and proliferative state.
TRiCBIO human endometrial organoid characterization data
View imageStage-dependent morphology: serial imaging within one culture workflow helps define time points for downstream assays.
TRiCBIO human endometrial organoid characterization data
View imageStage markers: immunofluorescence for ER, PR and FOXO1 adds phenotype context across culture stages for in vitro stage research and candidate-response assessment.
TRiCBIO human endometrial organoid characterization dataBegin with establishment and passage
Organoid formation after primary tissue processing and continued growth after passage document establishment and passaging performance; culture conditions and observation times are set for each sample.
Confirm epithelial identity and proliferation
Together, these readouts establish epithelial organization and a proliferative baseline before stage-directed or candidate-response studies.
- Source-tissue and organoid H&E
- E-cadherin epithelial profiling
- Ki67 proliferation profiling
Assess stage-dependent change
Serial morphology and immunofluorescence for ER, PR and FOXO1 compare proliferative, secretory and receptive culture stages for in vitro stage research and candidate-response assessment.
Define cycle context, conditions and endpoints
For a new study, define the cycle stage, hormonal or treatment conditions, replication plan and primary endpoints for candidate-response, inflammation or injury research.
INTERPRETATION
Epithelial identity and stage-related changes
These profiles guide model selection for candidate, hormonal, inflammatory and tissue-injury studies.
Follow-on studies can compare dose and time-course effects, with confirmation in independent samples.
Related models and services
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Study planning
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Model establishment, stage characterization or treatment-response studies
Sample context, cycle or hormone conditions, project goal and intended assays
Establishment and passage, epithelial identity checks and stage-associated morphology/marker profiling
Study images, assay results, method records, integrated analysis and study-design notes
Define the stage-directed or candidate-focused study
Cycle context, induction conditions, batch replication and functional endpoints for each new sample
PROJECT DISCUSSION
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Tell us which model features and readouts matter most. Our scientists will recommend the model, QC plan and study endpoints.