Candidate and exposure
Modality, concentration range, exposure time and control conditions.
Adjacent non-tumor liver organoid
Organoids from adjacent non-tumor liver tissue for epithelial-biology, drug-exposure and tumor-versus-adjacent-tissue studies.
CK19
Model source
Adjacent non-tumor liver tissue from surgical specimens
Culture / format
3D culture · Organoid 1.0 / 2.0 comparison
Supporting evidence
TRiCBIO adjacent non-tumor liver organoid culture and characterization
Model characterization
How the model is established and characterized
Culture morphology, histology and tissue-lineage markers establish the model profile and guide selection of subsequent efficacy, safety or functional endpoints.
View imageCulture and identity: serial P0 growth is shown with H&E; the panel shown is CK19-positive and SMA-negative.
Study data
View imageSerial culture: bright-field views at P0 days 0, 4, 8, 10, 14 and 17 show morphological change during establishment and guide culture-state selection for subsequent studies.
Study data
View imageHistology and identity: H&E shows tissue structure; the CK19-positive, SMA-negative panel supports liver-epithelial, injury and adjacent-tissue comparison studies.
Study dataStudy planning
From study objective to model and assay plan
Candidate exposure and the priority organ-safety or functional endpoints shape the model conditions, dosing and readouts.
Safety or functional focus
Priority organ-injury, efficacy or functional endpoints, together with the readouts needed to interpret them.
Model and deliverables
Required culture format, study batches, raw data, images and reporting needs.
Study design
Epithelial profiling and research applications of tumor-adjacent liver organoids
Derived from adjacent non-tumor liver tissue, this model combines culture morphology, histology and epithelial markers for liver epithelial biology, drug exposure, tissue injury and tumor-adjacent comparisons.
Review tissue and pathology
Record tumor adjacency, sampling region and available pathology.
Document culture and histology
Use serial culture, H&E and morphology to assess growth and epithelial structure.
Define identity with markers
Serial culture and H&E show model structure; the CK19-positive, SMA-negative panel adds epithelial identity to the model profile.
Design the intervention strategy
Select endpoints for drug exposure, injury or tumor-adjacent comparison.
Research applications
Recommended applications
- Liver epithelial biology
- Drug exposure and tissue injury
- Tumor-versus-adjacent-tissue comparison
Assays & QC
Organ identity, injury and functional readouts
Key markers
CK19
Available assays
- Bright-field imaging
- H&E
- IHC
- Morphology
QC approach
Serial morphology and H&E show tissue structure; the panel shown is CK19-positive and SMA-negative, adding epithelial identity to the model profile.
Study & delivery
Exposure design, organ-specific endpoints and reporting
Choose model establishment, culture or dosing studies. Typical deliverables include imaging, histology and a study report.
Related solutions
Related study options
PROJECT DISCUSSION
Discuss a liver organoid study
Candidate profile, expected exposure and priority organ risk determine dose conditions, controls and injury or functional endpoints for the adjacent non-tumor liver organoid.